Protocols

Three Dimensional Growth of MCF10A in Matrigel PDF icon 10A Morphogenesis_EL.pdf
DQ Collagen Assay in Matrigel PDF icon DQ Matrigel.doc.pdf
Seeding of MCF10A for 3D morphogenesis PDF icon Seeding_of_MCF10A_for_3D.doc.pdf
Generation of Collagen: Matrigel mixed Gel PDF icon Generation_of_Collagen.doc.pdf
LDH Assay (for quantification of 3D lysates) PDF icon LDH.doc.pdf
Ethidium Bromide staining of MCF 10A acini PDF icon EtBr staining_EL.doc.pdf
Collagen/Matrigel mixed cultures for MCF-10A cells PDF icon Collagen_EHS morphogenesis.pdf
Care and Passage of MCF-10A Cells In Monolayer Culture PDF icon Care_Passage_MCF_Matrigel.doc.pdf
Acrobat Immunofluorescence of Mammary Epithelial Acini PDF icon 3D IF Protocol_EL.doc.pdf
Freezing Cells PDF icon Freezing_Cells.doc.pdf
Overlay Three-Dimensional Culture of MCF-10A Cells on MatrigelTM PDF icon 3DimensionalCulture_of_MCF-10A.doc.pdf
Embedding Protocol for MCF-10A Frozen Sections PDF icon EmbeddingProtocoMCF_frozen.pdf
Staining Protocol for MCF-10A Frozen Sections PDF icon StainingProtocol_MCF_Frozen.pdf
Harvesting Protein Lysates from 3-D Acinar Cultures (for quantification of non-phosphorylated, intracellular, stable proteins) PDF icon HarvestingProteinLysates_Stable.doc.pdf
Acrobat Harvesting Protein Lysates from 3-D Acinar Cultures (for quantification of phospho-proteins, extracellular proteins, or quickly degraded proteins) PDF icon Harvesting Protein Lysates_QuicklyDegraded.doc.pdf
Harvesting RNA from 3-D Acinar Cultures PDF icon HarvestingRNA_3D_AcinarCultures.doc.pdf
Acrobat Ethidium Bromide Staining For Analysis of Cell Death during 3-D Culture PDF icon EthidiumBromideStainingAnalysisCellDeath.doc.pdf
Indirect Immunofluorescent Staining of MCF-10A Acini Cultured In Matrigel (Simplified) PDF icon ImmunofluorescentStainingMCF-10A_Acini_Simplified.doc.pdf
Indirect Immunofluorescent Staining of MCF-10A Acini Cultured In Matrigel (Detailed) PDF icon ImmunofluorescentStainingMCF-10A_Acini_Detailed.doc.pdf
Immunofluorescence Wash Buffer Recipe PDF icon BUFFER_RECIPES.doc.pdf
MCF-10A Media Recipes PDF icon MediaRecipesForMCF10ACells.pdf
Useful Antibodies And Fluorescent Reagents For The Analysis of MCF-10A Mammary Acini PDF icon UsefulAntibodiesAndFlourescentReagents.pdf