|
Three Dimensional Growth of MCF10A in Matrigel |
 10A Morphogenesis_EL.pdf |
|
DQ Collagen Assay in Matrigel |
DQ Matrigel.doc.pdf |
|
Seeding of MCF10A for 3D morphogenesis |
Seeding_of_MCF10A_for_3D.doc.pdf |
|
Generation of Collagen: Matrigel mixed Gel |
Generation_of_Collagen.doc.pdf |
|
LDH Assay (for quantification of 3D lysates) |
LDH.doc.pdf |
|
Ethidium Bromide staining of MCF 10A acini |
EtBr staining_EL.doc.pdf |
|
Collagen/Matrigel mixed cultures for MCF-10A cells |
Collagen_EHS morphogenesis.pdf |
|
Care and Passage of MCF-10A Cells In Monolayer Culture |
Care_Passage_MCF_Matrigel.doc.pdf |
|
Acrobat Immunofluorescence of Mammary Epithelial Acini |
3D IF Protocol_EL.doc.pdf |
|
Freezing Cells |
Freezing_Cells.doc.pdf |
|
Overlay Three-Dimensional Culture of MCF-10A Cells on MatrigelTM |
3DimensionalCulture_of_MCF-10A.doc.pdf |
|
Embedding Protocol for MCF-10A Frozen Sections |
EmbeddingProtocoMCF_frozen.pdf |
|
Staining Protocol for MCF-10A Frozen Sections |
StainingProtocol_MCF_Frozen.pdf |
|
Harvesting Protein Lysates from 3-D Acinar Cultures (for quantification of non-phosphorylated, intracellular, stable proteins) |
HarvestingProteinLysates_Stable.doc.pdf |
|
Acrobat Harvesting Protein Lysates from 3-D Acinar Cultures (for quantification of phospho-proteins, extracellular proteins, or quickly degraded proteins) |
Harvesting Protein Lysates_QuicklyDegraded.doc.pdf |
|
Harvesting RNA from 3-D Acinar Cultures |
HarvestingRNA_3D_AcinarCultures.doc.pdf |
|
Acrobat Ethidium Bromide Staining For Analysis of Cell Death during 3-D Culture |
EthidiumBromideStainingAnalysisCellDeath.doc.pdf |
|
Indirect Immunofluorescent Staining of MCF-10A Acini Cultured In Matrigel (Simplified) |
ImmunofluorescentStainingMCF-10A_Acini_Simplified.doc.pdf |
|
Indirect Immunofluorescent Staining of MCF-10A Acini Cultured In Matrigel (Detailed) |
ImmunofluorescentStainingMCF-10A_Acini_Detailed.doc.pdf |
|
Immunofluorescence Wash Buffer Recipe |
BUFFER_RECIPES.doc.pdf |
|
MCF-10A Media Recipes |
MediaRecipesForMCF10ACells.pdf |
|
Useful Antibodies And Fluorescent Reagents For The Analysis of MCF-10A Mammary Acini |
UsefulAntibodiesAndFlourescentReagents.pdf |
